【摘 要】
：
为了建立达氟沙星(DFLX)残留的免疫学检测方法,试验以前期获得的DFLX抗体和达氟沙星与卵清白蛋白的偶联物(DFLX-OVA)为基础,优化并建立了间接竞争ELISA检测方法。结果表明:该
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为了建立达氟沙星(DFLX)残留的免疫学检测方法,试验以前期获得的DFLX抗体和达氟沙星与卵清白蛋白的偶联物(DFLX-OVA)为基础,优化并建立了间接竞争ELISA检测方法。结果表明:该方法对DFLX最低检出限达29.24 ng/mL,且能对盐酸环丙沙星、氧氟沙星、沙拉沙星和恩诺沙星进行特异性识别。说明试验所建立的检测方法可用于DFLX药物残留的筛选检测。
To establish an immunological assay for residues of danofloxacin (DFLX), the assay was based on previously obtained DFLX antibodies and a conjugate of danofloxacin and ovalbumin (DFLX-OVA), optimizing and establishing indirect competition ELISA test method. The results showed that the detection limit of DFLX was 29.24 ng / mL. The method could specifically recognize ciprofloxacin hydrochloride, ofloxacin, sarafloxacin and enrofloxacin. Description Test established by the test method can be used for DFLX drug residue screening test.
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