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【目的】探讨蝉芩颗粒对神经生长因子(NGF)介导的PM2.5所致气道神经炎症的影响及其作用机制。【方法】将40只SD大鼠随机分为5组,即盐水对照组、PM2.5染毒组、抗NGF组、蝉芩颗粒组、惠菲宁组,每组8只。复制PM2.5大鼠气道滴注染毒模型。PM2.5染毒组大鼠染毒后给予等体积生理盐水灌胃,抗NGF组抗NGF干预染毒后给予生理盐水灌胃,蝉芩颗粒组染毒后予蝉芩颗粒(9.36 g·kg~(-1)·d~(-1))灌胃,惠菲宁组染毒后予惠菲宁糖浆(8 mL·kg~(-1)·d~(-1))灌胃,盐水对照组大鼠以生理盐水代替PM2.5气道灌注后给予等体积生理盐水灌胃,共2周。给药结束后,采用酶联免疫吸附(ELISA)法检测各组大鼠血清中P物质(SP)、降钙素基因相关肽(CGRP)、速激肽A(NKA)、速激肽B(NKB)、NGF的含量,采用免疫组化法检测各组大鼠肺组织、背根神经节中SP、NGF的表达,分别采用免疫印迹(Western blot)法及实时荧光定量逆转录—聚合酶链反应(RT-qPCR)法检测各组大鼠肺组织、背根神经节中NGF蛋白和m RNA的表达。【结果】与盐水对照组比较,染毒组大鼠血清中SP、CGRP、NKA、NKB、NGF值,肺组织NGF,背根神经节SP、NGF的免疫组化平均光密度值,肺组织中NGF蛋白和m RNA及背根神经节中NGF蛋白的表达水平均显著升高(P<0.05);与染毒组比较,抗NGF组、蝉芩颗粒组、惠菲宁组上述指标均显著降低(P<0.05),但3组比较,差异无统计学意义(P>0.05)。【结论】蝉芩颗粒可通过抑制NGF的表达减轻PM2.5所致大鼠气道神经源性炎症。
【Objective】 To investigate the effect and mechanism of Chanchuang Granule on NGF-mediated airway nerve inflammation induced by PM2.5. 【Methods】 Forty SD rats were randomly divided into 5 groups: saline control group, PM2.5-treated group, anti-NGF group, chishenqin granule group and Huifiningn group, with 8 rats in each group. Copy PM2.5 rat airway instillation model. Rats in PM2.5 group were given normal saline after intragastric administration, anti-NGF group were given anti-NGF intervention and saline was given intragastrically. After treated with Qichai granule group, the rats were treated with Chanqin granule (9.36 g · kg -1) ~ (-1) · d ~ (-1)) were given into the stomach and the Huifu Ning group was given gavagenol (8 mL · kg -1 · d -1) Rats in the control group were given saline instead of PM2.5 airway and then given an equal volume of normal saline for 2 weeks. After administration, serum SP, CGRP, NKA, tachykinin B (P <0.05) were detected by enzyme linked immunosorbent assay (ELISA) NGF and NGF were detected by immunohistochemistry. The expression of SP and NGF in lung tissue and dorsal root ganglion of rats in each group were detected by immunohistochemistry. Western blot and real-time quantitative reverse transcription-polymerase chain reaction (RT-qPCR) method was used to detect the expression of NGF protein and m RNA in the lung tissue and dorsal root ganglion of rats in each group. 【Results】 Compared with the saline control group, the mean optical density of SP, CGRP, NKA, NKB, NGF, NGF, SP and NGF in the serum of the rats in the treated group were significantly lower than those in the saline control group NGF protein, m RNA and NGF protein expression in dorsal root ganglion (P <0.05). Compared with the control group, the above indexes in anti-NGF group, CHIQIN granules group and Huifu Ning group were significantly decreased (P <0.05), but there was no significant difference between the three groups (P> 0.05). 【Conclusion】 Cici-qin granule can relieve airway neurogenic inflammation in rats induced by PM2.5 by inhibiting the expression of NGF.