研究化学物的经皮吸收已采用离体试验方法,评价皮肤屏障性质、化学物经皮吸收的理化过程及影响因素。通常用于经皮吸收离体试验的接受液,只提供生理的pH值和离子强度,以延缓皮角质细胞膜的变质。然而,近年已有学者提出,皮肤具有多种代谢功能及对外来物的解毒作用。因此,在采用离体试验评价化学物的经皮吸收时,同样需要研究母体化学物在经皮吸收过程中的代谢。为评价化学物在皮肤内的生物转化率,在进行离体试验时必须保持 Study of percutaneous absorption of chemicals has been used in vitro test methods to evaluate the nature of the skin barrier, chemical and physical absorption of the physical and chemical processes and influencing factors. Recipients commonly used for percutaneous absorption of in vitro tests only provide physiological pH and ionic strength to delay the deterioration of the cutin cell membrane. However, some scholars have proposed in recent years that the skin has a variety of metabolic functions and detoxification of foreign objects. Therefore, when using in vitro tests to assess the transdermal absorption of chemicals, it is also necessary to study the metabolism of the parent chemical during percutaneous absorption. To assess the rate of bioconversion of chemicals in the skin, it must be maintained during the in vitro test