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目的 :探讨肿瘤细胞在接受 γ射线照射后多核细胞的形成与细胞放射敏感性的关系。方法 :利用定时显微摄影装置对人膀胱癌细胞株 RT112和人胚胎肠上皮细胞株 I40 7在 8Gyγ射线照射后的细胞改变进行 2 40 h连续观察。结果 :两个细胞株受 γ射线照射后不仅出现细胞的凋亡和坏死 ,还有双核或多核细胞的形成。两个细胞株的双核或多核细胞的形成方式各不相同 ,I40 7主要是细胞融合 ,RT112则基本上是由于细胞分裂失败而形成 ,其双核或多核细胞的形成指数分别为 42 .9和5 4.7,除少数最终凋亡和坏死外 ,大部分持续生存直至观察结束 ,但由于未能进行有效的分裂 ,可视为增殖性死亡。而对照组细胞未发现双核或多核细胞的形成。结论 :实验结果证明不仅细胞凋亡和坏死影响着细胞的放射敏感性 ,多核细胞的形成也起着重要的作用。
Objective: To investigate the relationship between the formation of multinucleated cells and the radiosensitivity of tumor cells after receiving γ-ray irradiation. Methods: The cell changes of human bladder cancer cell line RT112 and human embryonic intestinal epithelial cell line I407 after 8 Gy γ-ray irradiation were observed continuously for 240 h using a time-lapse photomicrograph. RESULTS: Both cell lines were exposed to gamma rays not only apoptosis and necrosis of cells, but also the formation of binuclear or multinucleated cells. The formation of binuclear or multinuclear cells differed between the two cell lines, with I407 predominately cellular fusion and RT112 essentially due to cell division failure, with binucleated or multinucleated cells forming an index of 42.9 and 5, respectively 4.7. Except for a few final apoptotic and necrotic episodes, the majority survived until the end of observation, but could be considered as proliferative death due to failure to effectively divide. The control group cells found no dual or multi-nucleated cells. Conclusion: The experimental results show that not only apoptosis and necrosis affect the radiosensitivity of cells, but also the formation of multinucleated cells.