论文部分内容阅读
用以分析含半胱氨酸肽的方法有电泳法、柱层析法等。而离析含巯基肽,目前则只可在不溶性基质上共价结合或亲合吸附。 J.Y.张等提出一简便方法,包括先用一疏水生色团共价标记肽上的巯基,酶解后,用反相hplc分离。此种标记试剂可使含巯基肽比不含巯基肽的疏水性强,因此在洗脱时,保留时间长。而且因有生色团,在可见区可以监测。所用试剂系以二甲氨基偶氮苯胺为生色团,按下列方法合成。
Methods for analyzing cysteine-containing peptides include electrophoresis, column chromatography and the like. Segregation of thiol-containing peptides, currently only covalently bound or affinity-adsorbed on insoluble substrates. J.Y. Zhang et al propose a convenient method which involves first covalently labeling the thiol groups on a peptide with a hydrophobic chromophore. After enzymolysis, it is separated by reversed-phase hplc. Such a labeling reagent can make the thiol-containing peptide more hydrophobic than the non-thiol-containing peptide and thus retain a long retention time upon elution. And because of chromophores, in the visible area can be monitored. The reagent used was dimethylaminoazoaniline as a chromophore and was synthesized as follows.