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目的:用薄层扫描法对龙胆泻肝丸中马兜铃酸进行定量分析。方法:双波长反射法锯齿扫描,λ_S=395nm,λ_R=500nm;狭缝宽度:0.4 mm×0.4 mm;线性化器:SX=3;展开剂:苯-甲醇-36％醋酸(17:2:0.2)。结果:马兜铃酸标准曲线线性范围0.9～4.5μg(r=0.9998),平均回收率96.78％(n=6)。结论:方法准确,回收率好。
OBJECTIVE: To quantitatively analyze aristolochic acid in Longdanxiegan Pills by thin-layer scanning. Method: double-wavelength reflection method sawtooth scanning, λ_S = 395nm, λ_R = 500nm; slit width: 0.4mm × 0.4mm; linearizer: SX = 3; developing agent: benzene - methanol - 36% acetic acid (17:2: 0.2). Results: The linear range of aristolochic acid standard curve was 0.9-4.5μg (r=0.9998), and the average recovery rate was 96.78% (n=6). Conclusion: The method is accurate and the recovery rate is good.