按照Simoni等(1983)介绍的方法,在孕早期用直接法从绒毛制备胎儿的染色体时,作者遇到了困难。因为只能得到很少的分裂相,而且质量不好,尤其是显带质量不好。为了克服此困难,经改良后,建立了下述可重复的方法: 一、取5mg标本,放入含20%小牛血清的RPM1 According to the method described by Simoni et al. (1983), the authors encountered difficulties in preparing the fetus’s chromosome from the villus using the direct method during the first trimester of pregnancy. Because only get a few split phase, and the quality is not good, especially the quality of the band is not good. In order to overcome this difficulty, the improved and established the following repeatable method: First, take 5mg specimens into 20% fetal bovine serum RPM1