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目的建立超快速液相色谱(UFLC)法同时测定栀子中京尼平苷、京尼平1-β-D-龙胆双糖苷、京尼平苷酸和绿原酸的含量。方法采用Shim-Pack XR-ODS柱(75 mm×3.0 mm,2.2μm);流动相为0.1%甲酸(A)-乙腈(B),梯度洗脱(0~5 min,7%~12%B;5~11 min,12%~13%B);平衡时间为2 min;流速为0.4 m L·min~(-1);检测波长为240 nm;柱温为35℃。结果京尼平苷、京尼平1-β-D-龙胆双糖苷、京尼平苷酸和绿原酸分别在20~200μg·m L-1(r=0.999 9)、4.0~40μg·m L~(-1)(r=0.999 7)、4.0~40μg·m L~(-1)(r=0.999 9)和4.0~40μg·m L~(-1)(r=0.999 9)浓度范围内与峰面积呈良好的线性关系;平均回收率分别为99.0%、99.6%、96.9%和98.7%(n=9)。结论该方法简便、快速,重复性良好,可为栀子药材的质量控制提供依据。
Objective To establish an ultra fast liquid chromatography (UFLC) method for simultaneous determination of geniposide, genipin 1-β-D-gentiobioside, geniposidic acid and chlorogenic acid in Fructus Gardeniae. Methods A Shim-Pack XR-ODS column (75 mm × 3.0 mm, 2.2 μm) was used. The mobile phase consisted of 0.1% formic acid (A) ; 5 to 11 min, 12% to 13% B); the equilibration time was 2 min; the flow rate was 0.4 m L · min -1; the detection wavelength was 240 nm; Results The concentrations of geniposide, genipin 1-β-D-gentiobioside, geniposidic acid and chlorogenic acid were in the ranges of 20 ~ 200μg · m L -1 (r = 0.999 9), 4.0 ~ 40μg · (r = 0.999 7), 4.0 ~ 40μg · m L -1 (r = 0.999 9) and 4.0 ~ 40μg · m L -1 (r = 0.999 9) The linear range of the peak area was 99.0%, 99.6%, 96.9% and 98.7%, respectively (n = 9). Conclusion The method is simple, rapid and reproducible, which can provide the basis for the quality control of Fructus Gardeniae.