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目的 探讨中国人群中盐皮质激素受体基因 (MR)外显子 3上A76 0G多态性与妊高征的相关性。方法 以人外周血DNA为模板 ,通过聚合酶链式反应 -等位特异性寡核苷酸杂交 (PCR -ASO)方法测定 95例随机妇女、10 1例正常孕妇、6 0例妊高征妇女的MR外显子 3上A76 0G多态性频率。结果 MR外显子 3上A76 0G多态性位点中A等位基因和C等位基因在正常孕妇组中分别为 83.17%与 16 .83% ,在妊高征组中分别为 84 .17%与 15 .83% ,在随机人群组中分别为 86 .84 %与 13.16 %。结论 中国人群中MR基因外显子 3上存在A76 0G多态性 ,该多态性与妊高征不存在相关性。
Objective To investigate the association between A76 0G polymorphism in the mineralocorticoid receptor gene (MR) exon 3 and pregnancy-induced hypertension in Chinese population. Methods 95 cases of randomized women, 101 normal pregnant women and 60 PIH women were detected by polymerase chain reaction-allele-specific oligonucleotide hybridization (PCR-ASO) method using human peripheral blood DNA as a template. Of the A76 0G polymorphism on MR exon 3. Results The A allele and C allele of A76 0G polymorphism in exon 3 of MR were 83.17% and 16.83% in normal pregnant women and 84.17% in pregnant women with PIH respectively % And 15.83%, respectively, in the random cohort of 86.84% and 13.16%. Conclusion There is A76 0G polymorphism in MR gene exon 3 in Chinese population, which is not associated with PIH.