论文部分内容阅读
用YC2(高杆)×YF1(矮杆)和YC1(高杆)×YF1(矮杆)组合衍生的2个F2代群体,对蓖麻株高性状进行相关、回归和QTL定位分析。结果表明,株高与主穗位高、主茎节长和主茎茎粗之间显著正相关,但与主茎节数不相关;主穗位高与主茎节数、主茎节长和主茎茎粗之间呈显著正相关;主茎节数与主茎节长之间呈显著负相关。利用QTLNetwork 2.0软件在YC2×YF1的F2群体中检测到株高、主穗位高、主茎节数、主茎节长和主茎茎粗的5、4、6、3和2个QTL,分别解释了45.9%、45.3%、66.1%、55.4%和12.6%的总变异。在YC1×YF1的F2群体中检测到3、4、5、1和2个上述5性状的QTL,分别解释了26.0%、25.5%、35.4%、37.4%和7.6%的总变异。证明QTL间的“一因多效”和连锁是株高、主穗位高和主茎节长之间高度相关的遗传基础,加性效应是株高、主穗位高和主茎节长的主要遗传组分,互作效应是主茎节数和主茎茎粗的主要遗传组分。建议育种上将主穗位高和主茎节长作为株高早期选择和预测的间接指标,并将多节数和短节间作为高产育种的主攻方向。
Two F2 populations derived from a combination of YC2 (tall rod) × YF1 (dwarf rod) and YC1 (tall rod) × YF1 (dwarf rod) were used for correlation analysis, regression and QTL mapping of castor plant height traits. The results showed that there was a significant positive correlation between the plant height and the height of main ear, the length of main stem and the diameter of main stem, but not with the number of main stems. The height of main ear was related to the number of main stems, There was a significant positive correlation between stem diameter and stem diameter. The number of main stems was negatively correlated with the length of main stems. QTLNetwork 2.0 software was used to detect 5, 4, 6, 3 and 2 QTLs of plant height, main ear height, main stem number, main stem length and main stem diameter in YC2 × YF1 F2 population respectively Explained 45.9%, 45.3%, 66.1%, 55.4% and 12.6% of the total variance. Three, four, five, one and two QTLs for the above five traits were detected in the YC1 × YF1 F2 population, accounting for 26.0%, 25.5%, 35.4%, 37.4% and 7.6% of the total variance, respectively. It was proved that there was a high genetic correlation between plant height, height of main ear and length of main stem in QTLs. The additive effects were plant height, height of main ear and main stem The main genetic component of long, interaction effect is the main stem section and the main stem diameter of the main genetic components. It is suggested that the main ear height and main stem length should be used as an indirect indicator of plant height selection and prediction, and the multi-node number and inter-node spacing should be taken as the main direction of high-yielding breeding.