雌、雄弓背青鳉(Oryzias curvinotus)肝脏转录组比较分析

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为发掘弓背青鳉(Oryzias curvinotus)肝脏功能基因、开发环境检测标记基因,本研究采用RNA-Seq技术分别测定了性成熟雌、雄弓背青鳉肝脏转录组,并获得80095044和87043984条clean reads,合并拼接出49912个unigenes(N50=2394bp)。基因表达差异分析显示在雌、雄弓背青鳉肝脏组织中存在571个差异基因(DEGs),其中364个在雄鱼中高表达,207个在雌鱼中高表达。GO和KEGG分析表明差异基因主要参与激素合成、免疫反应、氧化还原反应及卵黄发生等生物学
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