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ＣａＭ为重要的调控蛋白，Ｃａ２＋ＡＴＰａｓｅ则是重要的ＣａＭ结合蛋白，它和ＣａＭ一起构成信号传递通路中的重要组成部分，是功能上密切相关的两个基因。以生物素标记这两个基因的ｃＤＮＡ为探针，用原位杂交技术将它们定位到了水稻（ＯｒｙｚａｓａｔｉｖａＬ．）染色体上，检出率为６．１８％。ＣａＭ和Ｃａ２＋ＡＴＰａｓｅ基因被分别定位于第５染色体长臂的末端和第５染色体短臂紧靠着丝粒处。所检出染色体的臂比及标准差分别为１．７９±０．０６和１．９１±０．０８。二者在遗传图中相距较近，而在基因组物理图中位于同一染色体上不同的染色体臂上，说明基因的遗传图和物理图之间存在差异。并对短片段，低拷贝或单基因的技术进行了讨论。
CaM is an important regulatory protein, and Ca2 + -ATPase is an important CaM binding protein. CaM binds with CaM and forms an important part of the signal transduction pathway. It is two closely related genes. Using biotinylated cDNAs of these two genes as probes, they were mapped to the Oryza sativa L. chromosome by in situ hybridization with a detection rate of 6.18%. The CaM and Ca2 + -ATPase genes were located at the end of the long arm of chromosome 5 and the short arm of chromosome 5 close to the centromere. The arm ratios and standard deviations of the detected chromosomes were 1.79 ± 0.06 and 1.91 ± 0.08, respectively. The two are close to each other in the genetic map, and on the different chromosomal arms of the same chromosome in the genome physics map, there is a difference between the genetic map and the physical map of the gene. And the technology of short fragment, low copy or single gene are discussed.