Radioiodide uptake in melanoma cells after transfer of human NaI symporter gene

来源 :核技术 | 被引量 : 0次 | 上传用户:ghanfeng
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
To obtain human sodium/iodide symporter gene cDNA for studying its potential ability as a radioiodide treatment for melanoma, the hNIS gene cDNA was amplified with total RNA from human thyroid tissue by RT-PCR.The hNIS cDNA was inserted into cloning vector pUCm-T and subcloned into eukaryotic expression vector pc-DNA3.The pc-DNA3-hNIS and pc-DNA3 were transduced into melanoma cells (B 16) by electroporation, and two cell lines termed B 16-A and B16-B respectively were established. The uptake and efflux of iodide was examined in vitro. The three cell lines (B16-A, B16-B, B16) were injected subcutaneously into the right flank of C57 mice. Biodistribution study and tumor imaging were performed when the tumor reached approximately 10mm in diameter. The cloned hNIS cDNA sequence was identical with the published sequence. Two novel cell lines named 16-A containing pc-DNA3-hNIS and B16-B containing pc-DNA3 only were established. The resultant cell line B16-A accumulated 17and 19 times more radioiodide in vitro than B 16 and B 16-B respectively. The iodide uptake reached the half-maximal level within 10 min, and reached a plateau at 30 min. The efflux of iodide was also rapid (T1/2cff=10min). The imaging shows in vivo uptake in expected sites including the salivary glands, thyroid, stomach, and hNIS-transduced tumor,whereas the nontransduced tumor was not visualized. The %ID/g of B 16-A tumors at 1, 2, 4, 12, and 24h after injection of 125I were 12.22±0.71, 10.91±0.72, 8.73±0.99, 1.24±0.29, and 0.19±0.03, respectively, which were signifi cantly higher percentages than those for controlling tumors, p<0.01. However, biologic T1/2 was about 6 h. Our preliminary data indicate that the transduction of the hNIS gene per se is sufficient to induce iodide transport in melanoma cells both in vitro and in vivo, but T1/2eff is short.
其他文献
To prepare monoclonal antibody specific to epidermal growth factor receptor (EGFR) intracellular domain, its gene was amplified from total RNA of A431 cell by R
To understand the antagonistic mechanism of the broad spectrum antagonistic Enterobacter cloacae B8,Tn5 transposon-mediated mutagenesis is performed using suici
掌握科技情报对当今信息时代下发表科研最新成果的科技期刊来说是举足轻重的。本文在阐述信息检索知识在科技期刊编辑工作中重要性的基础上,介绍了信息检索的基本知识及检索
Recombinant human prolactin (rhPRL) was administered to huPBL-SCID mice to determine its effects on human immunologic reconsfitution and function. The huPBL-SCI
The adult brain has been thought traditionally as a structure with a very limited regenerative capacity. It is now evident that neurogenesis in adult mammalian
Objective: To observe and evaluate the therapeutic effect of anti-tuberculosis (anti-TB) chemicals and Compound Astragalus Capsule (CAC) in combinedly treating
Hydroxyapatite ceramics (HA) has been proved to be excellent in biocompatibility and bioactivity. However, limited information is available concerning how HA ce
Objective To investigate the cellular effects of cigarette smoke extract (CSE) on primarily cultured human umbilical vein endothelial cells (HUVEC). Methods The
目前,市场竞争越来越剧烈,各种建材价格也呈现出不断上扬的趋势,从而对施工单位的利润产生了不利的影响,随着施工单位所得利润的不断下降,成本控制与管理引起了建筑单位的普
本文介绍柴油机在使用过程中,常出现冷却液减少.那么引起柴油机冷却液减少有那些原因;以及如何诊断,本文作了详细说明.