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目的:研究妊娠期妇女子宫NK细胞(uNK细胞)与外周血NK细胞(pNK细胞)表面NKG2A和NKG2D及其相应配体的表达,探讨uNK细胞表面NKG2A和NKG2D的不平衡表达与母胎界面所形成的免疫耐受关系。方法:采用流式细胞术检测30例孕6～9周的正常妊娠妇女uNK细胞和pNK细胞NKG2A、NKG2D的表达状况;RTPCR技术检测绒毛膜组织HLAE、MICA的表达。结果:子宫NK细胞NKG2A的表达显著高于外周血NK细胞,二者分别为(97.86±1.75)%与(33.35±10.92)%;子宫NK细胞NKG2D的表达水平与外周血NK细胞相近,分别为(93.21±4.52)%与(97.80±1.72)%,滋养层组织仅检测到HLAEmRNA的表达。结论:妊娠期子宫NK细胞表面高表达抑制性受体NKG2A,同时滋养层组织表达相应的配体HLAE,这可能是维持母胎界面免疫耐受的重要因素。
OBJECTIVE: To study the expression of NKG2A and NKG2D and their corresponding ligands on uterine NK cells (uNK cells) and peripheral blood NK cells (pNK cells) in pregnant women, and to investigate the imbalanced expression of NKG2A and NKG2D on uNK cells and the formation of maternal-fetal interface Immune tolerance relationship. Methods: The expression of NKG2A and NKG2D in uNK cells and pNK cells in 30 pregnant women with normal pregnancy from 6 to 9 weeks were detected by flow cytometry. The expressions of HLAE and MICA in chorionic tissues were detected by RTPCR. Results: The expression of NKG2A in uterus NK cells was significantly higher than that in peripheral blood NK cells (97.86 ± 1.75)% and (33.35 ± 10.92)%, respectively. The expression level of NKG2D in uterine NK cells was similar to that of peripheral blood NK cells (93.21 ± 4.52)% and (97.80 ± 1.72)% respectively. Trophoblast tissue only detected the expression of HLAE mRNA. CONCLUSION: NKG2A is highly expressed on the surface of uterine NK cells in pregnancy and HLAE is expressed in trophoblast, which may be an important factor in maintaining immune tolerance of maternal-fetal interface.