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从木耳(Auricularia auricula)和毛木耳(A.polytricha)的同一子实体弹射、分离30个单孢子并发育成单核菌丝体,各自分成3组,以10×10方式进行单核体两两配对。取两配对单核体交结处菌丝体块到新的平板上继续发育并插入无菌的盖玻片让其菌丝爬上。后利用双苯并咪唑(Hoechst 33258)染色,在萤光显微镜下逐块检查配对后菌丝体细胞中核的数目。如果出现双核,再加以检查锁状联合以验证,则为配对亲和。不亲和者仍为单核。根据配对行为进行不亲和因子分配决定其交配型。检测结果表明,木耳和毛木耳担孢子的性别是由一对遗传因子A.a所控制。属典型性二极性(bipolar)异宗结合。
Ejection from the same fruiting body of Auricularia auricula and A. polytricha separated 30 monospores and developed into mononuclear mycelia, each divided into 3 groups, each pair of mononuclear bodies was paired in a 10 × 10 manner . Take two pairs of mononuclear intercourse at the mycelium block to a new plate to continue the development and insertion of sterile cover glass to let mycelium climb. After staining with bisbenzimidazole (Hoechst 33258), the number of nuclei in mycoplasma cells after mating was examined under a fluorescence microscope block by block. If binuclear, and then check the lock for verification, then matching affinity. Inconsistent people are still mononuclear. Affinity factor distribution according to the pairing behavior to determine its mating type. Test results show that the fungus and fungus spores of the sexes are controlled by a pair of genetic factors A. a. Is a typical bipolar (bipolar) heterogeneous combination.