论文部分内容阅读
目的建立一种胸部撞击致肺挫伤的大鼠模型。方法将84只SD大鼠随机分成7组(n=12),采用自制的胸部撞击装置,用不同撞击能量(2.7、2.0、1.8、1.5、1.2、0.9和0J)对大鼠胸部进行撞击造成双侧肺损伤。监测撞击前后大鼠循环和呼吸功能变化。撞击120min后处死大鼠,开胸行创伤评分,观察心肺组织形态学变化,并进行病理学评分,计算肺组织湿干比和肺泡灌洗液蛋白含量。结果用1.2J能量撞击大鼠胸部可致理想的双侧肺损伤而无明显心脏损伤,动物死亡率较低。结论采用自制胸部撞击装置建立的大鼠双侧肺损伤模型可以较好地模拟胸部撞击后双侧肺挫伤伤情,可用1.2J的撞击能量进行后续实验研究。
Objective To establish a rat model of pulmonary contusion induced by thorax impact. Methods Totally 84 SD rats were randomly divided into 7 groups (n = 12). The rats were impacted with different impact energies (2.7, 2.0, 1.8, 1.5, 1.2, 0.9 and 0J) Bilateral lung injury. Monitor changes in rat circulatory and respiratory functions before and after impact. The rats were sacrificed 120min after impact, the traumatic injury score of thoracotomy, morphological changes of the heart and lung were observed, and the pathological score was calculated. The wet-dry ratio of lung tissue and the protein content of bronchoalveolar lavage fluid were calculated. Results With 1.2J energy impact on the chest in rats can be caused by the ideal bilateral lung injury without significant heart damage, animal mortality was low. Conclusions Bilateral lung injury model established by self-made chest impact device can simulate the bilateral pulmonary contusion injury well after chest impact. The impact energy of 1.2 J can be used for subsequent experimental study.