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炭疽病是柑橘的主要真菌性病害之一。2007年春,广东省德庆县名优柑橘品种贡柑炭疽病暴发流行。为了明确该县及广东省其他地区柑橘炭疽病菌的种类,为防治提供依据,对采集自广东省6个地区柑橘属10个栽培品种上的炭疽病样本进行病原菌分离,共获得柑橘炭疽病菌单孢菌株75株,对其中10株代表性的菌株进行了种类鉴定。通过培养性状和形态学特征观测、核糖体DNA(rDNA)内转录间区(ITS)序列分析、ITS区特异性引物PCR检测和系统发育关系比较等方面的研究,结果表明:10个柑橘炭疽病菌菌株均为盘长孢状刺盘孢Colletotrichum gloeosporioides,未发现国际上其他国家报道的严重危害柑橘花器和幼果部位的柑橘花后落果病病原菌——尖刺盘孢C.acutatum。
Anthracnose is one of the major citrus fungal diseases. The spring of 2007, Deqing County, Guangdong Province famous orange citrus anthracnose outbreak popular. In order to clarify the species of citrus anthracnose in the county and other areas in Guangdong Province, anthrax samples collected from 10 cultivars of Citrus in six areas of Guangdong Province were isolated for pathogenic bacteria. Strain 75 strains, of which 10 representative strains were identified. Through the observation of traits and morphological characteristics, ITS sequence analysis of ribosomal DNA (rDNA), PCR detection of ITS region-specific primers and comparison of phylogenetic relationships, the results showed that 10 citrus anthracnose The strains were all Colletotrichum gloeosporioides. No other internationally reported C. citrullium, a pathogen of citrus orchard fall fruit disease, was severely endangered in citrus flowers and young fruits.