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目的探讨TNF-α启动子区基因多态性是否与宿主感染乙型肝炎病毒(hepatitis Bvirus,HBV)后形成不同的结局相关联。方法采用病例-对照研究方法,以362例慢性乙型肝炎患者作为病例组,212例乙型肝炎病毒自限性感染者作为对照组,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法对TNF-α基因启动子区-238G/A、-857C/T、-863C/A位点进行基因分型。结果慢性乙肝组携带TNF-α-238GA基因型36例(10.0%)、-857CC基因型289例(79.8%),自限性感染组分别为-238GA基因型11例(5.2%)、-857CC基因型149例(70.3%),慢性乙肝组均显著高于自限性感染组(P<0.05)。3个位点组成的单体型-238G/-857C/-863A的频率在慢性乙肝组显著高于HBV自限感染组(64.36%vs.58.26%)。单体型-238G/-857T/-863A在慢性乙肝组显著低于HBV自限感染组(7.32%vs.12.26%)。结论 TNF-α启动子区基因多态性可能与宿主感染HBV后形成慢性化结局相关联。
Objective To investigate whether the genetic polymorphism of TNF-α promoter region is associated with the formation of different outcomes in host infected with hepatitis B virus (HBV). Methods A case-control study was conducted in 362 patients with chronic hepatitis B and 212 patients with hepatitis B virus self-limited infection as control group. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) -RFLP) were used to genotype the -238G / A, -857C / T and -863C / A sites of TNF-α gene promoter region. Results There were 36 cases (10.0%) of TNF-α-238GA genotypes and 289 cases (79.8%) of 857CC genotypes in chronic hepatitis B group, and 11 cases (-2.28% 149 genotypes (70.3%) and chronic hepatitis B group were significantly higher than the self-limited infection group (P <0.05). The frequencies of haplotype-238G / -857C / -863A at the three loci were significantly higher in patients with chronic hepatitis B than in those with HBV self-limited infection (64.36% vs.58.26%). The haplotype-238G / -857T / -863A was significantly lower in the chronic hepatitis B group than in the HBV self-limiting infection group (7.32% vs. 12.26%). Conclusion The gene polymorphism of TNF-α promoter may be associated with chronic host-infected HBV infection.