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目的研究铜绿假单胞菌肺部感染与胸腺细胞凋亡的关系及肿瘤坏死因子（ＴＮＦ）介导机制。方法复制铜绿假单胞菌肺部感染动物模型，动态检测感染２４小时内血清，支气管肺泡灌洗液（ＢＡＬＦ）中ＴＮＦ及肺病理变化，同时采用碘化丙啶染色ＤＮＡ含量分析法观察胸腺细胞凋亡情况。结果发现小鼠吸入铜绿假单胞菌３小时后细菌学和病理学检查证实肺部有炎症发生，血清和ＢＡＬＦ中ＴＮＦ开始升高，同时胸腺细胞出现凋亡。在炎症过程中，ＢＡＬＦ及血清中ＴＮＦ升高与胸腺细胞凋亡发生率在动力学基本平行。结论铜绿假单胞菌肺部感染及菌血症可引起胸腺细胞发生凋亡，ＴＮＦ在细菌感染引起胸腺细胞凋亡中具有重要作用
Objective To study the relationship between pulmonary infection of Pseudomonas aeruginosa and apoptosis of thymus cells and tumor necrosis factor (TNF) mediated mechanism. Methods The animal model of Pseudomonas aeruginosa lung infection was replicated. TNF and pulmonary pathological changes in serum and bronchoalveolar lavage fluid (BALF) were detected dynamically within 24 hours after infection. Meanwhile, the content of thymus Apoptosis. Three hours after inhalation of Pseudomonas aeruginosa in mice, bacteriological and pathological examination revealed inflammation in the lungs. TNF levels in serum and BALF began to increase, and apoptosis of thymocytes occurred. During the inflammatory process, the increase of TNF in BALF and serum and the incidence of thymus apoptosis are basically in parallel kinetics. Conclusions Pseudomonas aeruginosa pulmonary infection and bacteremia can induce thymocyte apoptosis, and TNF plays an important role in the apoptosis of thymocytes caused by bacterial infection