Lipotransfer has become a powerful regenerative tool, largely because of its cellular components, the stromal vascular fraction (SVF). However, the clinical separation of cells with collagenase is strictly legislated. In 2017, Yao et al. postulated a novel fat-derived product mechanically concentrating SVF cells and an extracellular matrix (ECM) and named it stromal vascular fraction gel (SVF-gel). This review discussed the protocol of SVF-gel and its component as well as its inner structure. The histologic examination and the retention rate after the transplantation of SVF-gel were also rendered. Moreover, we summed up the rejuvenating and regenerative use of SVF-gel and introduced its possible mechanism.