论文部分内容阅读
AIM: To investigate the possibility that a novel α-helix-defective mutant of stromal cell-derived factor-1α (SDF- 1α) (SDF-1/54R) acts as an antagonist of CXC chemokine receptor 4 (CXCR4). METHODS: According to the genetic sequence of natural SDF-1α, a recombinant α-helix-defective mutant of SDF-1α was designed and some biologic characteristics of this mutant were demonstrated. The migration of Jurkat cells was assessed with chemo- tactic assay. ERK phosphorylation was analyzed by Western blot with a specific anti-phospho-ERK1/2 antibody. Intracellular calcium influx was examined by flow cytometer with a calcium indicator dye Fluo-3AM. The CXCR4 on the cell surface was detected by flow cytometer with a PE conjoined anti-human CXCR4 antibody. RESULTS: Compared with native SDF-1α, SDF-1/54R displayed apparent decrease in chemotactic ability, ERK1/2 activation, and intracellular calcium influx in Jurkat cells. However, the binding to CXCR4 and inducing CXCR4 internalization of SDF-1/54R did not change outstandingly. Moreover, a competitive inhibitory effect of SDF-1/54R on the migration of Jurkat cells induced by native SDF-1α was confirmed. CONCLUSION: α-helix-defective mutant of SDF-1α, SDF-1/54R that remained both the N-terminus and the central β-sheet region, decreased SDF-1α-medi- ated signaling and chemotaxis but did not influence CXCR4 internalization, which suggested that SDF-1/54R might be developed as an anti-CHIV inhibitor with high biological potency and low side-effect.
AIM: To investigate the possibility that a novel α-helix-defective mutant of stromal cell-derived factor-1α (SDF-1α) (SDF-1 / 54R) acts as an antagonist of CXC chemokine receptor 4 (CXCR4). METHODS: According to the genetic sequence of natural SDF-1α, a recombinant α-helix-defective mutant of SDF-1α was designed and some biologic characteristics of this mutant were demonstrated. The migration of Jurkat cells was evaluated with this mutant with ERK phosphorylation was analyzed by Western blot with a specific anti-phospho-ERK1 / 2 antibody. Intracellular calcium influx was examined by flow cytometer with a calcium indicator dye Fluo-3AM. The CXCR4 on the cell surface was detected by flow cytometer with a PE conjoined anti -human CXCR4 antibody. RESULTS: Compared with native SDF-1α, SDF-1 / 54R displayed apparent decrease in chemotactic ability, ERK1 / 2 activation, and intracellular calcium influx in Jurkat cells. However, the binding to CXCR4 and inducing CXCR4 internalization of SDF-1 / 54R on the migration of Jurkat cells by native SDF-1α was confirmed. CONCLUSION: α-helix-defective mutant of SDF-1α , SDF-1 / 54R that stands both the N-terminus and the central β-sheet region, decreased SDF-1α-medi- ated signaling and chemotaxis but did not affect CXCR4 internalization, which suggested that SDF-1 / 54R might be developed as an anti-CHIV inhibitor with high biological potency and low side-effect.