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用随机扩增的多态性ＤＮＡ（ＲＡＰＤ）技术对螺原体３个血清组中的４个菌株进行ＤＮＡ多态性研究。ＰＣＲ扩增引物选自Ｏｐｅｒｏｎ随机引物试剂盒中Ｉ、Ｊ、Ｋ、Ｌ、Ｍ、Ｎ组的１２０个引物。４个菌株为２３６（第Ⅲ血清组），ＣＲ１和ＣＨ９１１（第Ｉ血清组第２亚组），ＣＣＨ（第ⅩⅩⅣ血清组）。在１２０个引物中有２１个能在４种菌株ＤＮＡ模板上扩增出ＤＮＡ条带，其中在４种模板上皆可扩增出条带的引物有９个。电泳结果显示不同血清组菌株间ＤＮＡ多态性丰富，而同一血清组中菌株的ＤＮＡ扩增图谱差异微小。
DNA polymorphisms of 4 strains of 3 serogroups of spirochetes were studied using random amplified polymorphic DNA (RAPD) technique. The PCR amplification primers were selected from 120 primers in I, J, K, L, M, N groups in the Operon Random Primer Kit. Four strains of 23 6 (group Ⅲ serum), CR 1 and CH 91 1 (the first group of serum subgroup 2), CCH (Ⅹ Ⅹ Ⅳ serum group). Of the 120 primers, 21 were capable of amplifying DNA bands on 4 strains of DNA templates, of which 9 were primers capable of amplifying on all 4 types of template. The results of electrophoresis showed that there was abundant DNA polymorphism among strains of different serogroups, but there was no significant difference between the DNA amplifications in the same serogroups.