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目的:观察口服卡介菌对实验性变态反应性脑脊髓炎(EAE)的治疗效果。方法:制备EAE大鼠模型,随机分为BCG高、中、低剂量组和PBS对照组,每组各15只,对大鼠治疗后的临床症状及病理组织学进行评估,提取脾脏淋巴细胞,流式细胞术检测T淋巴细胞亚群,3H-TdR掺入法检测淋巴细胞增殖能力。结果:BCG组EAE大鼠与对照组相比,临床症状减轻,发病时间延迟,炎性细胞浸润数减少;急性期,口服BCG各组CD4+、CD8+T细胞的数量随剂量增加而增加,缓解期CD4+、CD8+T细胞数量减少;口服BCG可促进EAE大鼠T淋巴细胞增殖能力;高、中剂量组上述变化均较其它分组明显。结论:口服BCG可很好的诱导免疫耐受,延迟EAE发病,减轻炎症反应,改善临床症状。
Objective: To observe the therapeutic effect of oral BCG on experimental allergic encephalomyelitis (EAE). Methods: EAE rat models were prepared and randomly divided into BCG high, medium and low dose groups and PBS control group, 15 rats in each group. The clinical symptoms and histopathology of rats were evaluated, and the spleen lymphocytes were extracted, Flow cytometry was used to detect T lymphocyte subsets and 3H-TdR incorporation assay was used to detect the proliferation of lymphocytes. Results: Compared with the control group, the EAE rats in BCG group showed less clinical symptoms, delayed onset of symptoms and decreased inflammatory cell infiltration. In the acute phase, the number of CD4 + and CD8 + T cells in BCG increased with the increase of dose The number of CD4 +, CD8 + T cells decreased; oral administration of BCG can promote the proliferation of T lymphocytes in EAE rats; the high and medium dose groups were significantly higher than the other groups. Conclusion: Oral administration of BCG can well induce immune tolerance, delay the onset of EAE, reduce inflammation and improve clinical symptoms.