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目的:研究前列腺癌病灶内G蛋白偶联受体C6A(G protein-coupled receptor class C group 6member A,GPRC6A)和蛋白激酶Cζ(Protein Kinase Czeta,PKCzeta)表达水平与细胞增殖、上皮间质转化(EMT)的关系。方法:选择2014年6月~2017年3月期间在我院接受手术切除治疗的前列腺癌患者以及良性前列腺增生患者,留取前列腺癌患者的前列腺癌病灶、癌旁病灶适量,检测病灶中GPRC6A、PKCzeta、细胞增殖基因、EMT基因的表达量。结果:前列腺癌病灶、癌旁病灶中GPRC6A、Survivin、SRSF1、Bcl-xl、N-cadherin、Vimentin的表达量显著高于良性前列腺增生病灶,PKCzeta、Caspase-3、Caspase-9、Apaf-1、E-cadherin、CK5/6的表达量显著低于良性前列腺增生病灶;PKCzeta低表达的前列腺癌病灶中Survivin、SRSF1、Bcl-xl的表达量显著高于PKCzeta高表达的前列腺癌病灶,Caspase-3、Caspase-9、Apaf-1的表达量显著低于PKCzeta高表达的前列腺癌病灶;GPRC6A低表达的前列腺癌病灶中E-cadherin、CK5/6的表达量显著高于GPRC6A高表达的前列腺癌病灶,N-cadherin、Vimentin的表达量显著低于GPRC6A高表达的前列腺癌病灶。结论:前列腺癌病灶内高表达的GPRC6A和低表达的PKCzeta分别能够促进细胞EMT和增殖。
OBJECTIVE: To study the relationship between the expression of G protein-coupled receptor class C group 6member A (GPRC6A) and protein kinase C zeta (PKCzeta) and the cell proliferation and epithelial-mesenchymal transition in prostatic cancer EMT) relationship. Methods: Prostate cancer patients and benign prostatic hyperplasia patients undergoing surgical resection in our hospital from June 2014 to March 2017 were enrolled in this study. Prostate cancer lesions, paraquat lesions were collected from patients with prostate cancer, GPRC6A, PKCzeta, cell proliferation gene, EMT gene expression. Results: The expression of GPRC6A, Survivin, SRSF1, Bcl-xl, N-cadherin and Vimentin were significantly higher in benign prostatic hyperplasia, benign prostatic hyperplasia, PKCzeta, Caspase-3, Caspase-9, Apaf- The expression of E-cadherin and CK5 / 6 was significantly lower in benign prostatic hyperplasia lesions than in benign prostatic hyperplasia lesions. The expression of Survivin, SRSF1 and Bcl-xl in PKCzeta-overexpressing prostate cancer was significantly higher than that in PKCzeta-overexpressing prostate cancer and Caspase-3 , And the expressions of Caspase-9 and Apaf-1 were significantly lower in PKCzeta-overexpressing prostate cancer than in those with GPRC6A; the expression of E-cadherin and CK5 / 6 was significantly higher in the lesions with low GPRC6A expression than in those with high expression of GPRC6A , N-cadherin, Vimentin expression was significantly lower than the high expression of GPRC6A prostate cancer lesions. Conclusion: High expression of GPRC6A and low expression of PKCzeta in prostatic cancer lesions can promote EMT and proliferation respectively.