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目的探讨量子点CdSe及2种不同粒径的CdTe对L 929细胞增殖能力、氧化损伤和凋亡的影响。方法四甲基偶氮噻唑蓝(MTT)比色法检测3种量子点对L 929细胞增殖活力的影响;检测染毒后L 929细胞中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽-过氧化物酶(GSH-Px)和羟自由基(.OH)的含量及细胞凋亡情况。结果染毒24 h后半数抑制浓度(IC50)为:2.2 nm CdSe 27.14μg/mL,2 nm CdTe 28.28μg/mL,3.5 nm CdTe 79.30μg/mL;不同粒径的2种CdTe比较,14μg/mL剂量时,GSH-Px和.OH指标差异有统计学意义(P<0.05);3种量子点的高剂量组细胞凋亡率与对照组比较差异有统计学意义(P<0.05),不同粒径的2种CdTe比较,各剂量组差异均有统计学意义(P<0.05)。结论量子点可以抑制小鼠成纤维细胞增殖能力,并发生氧化损伤作用,诱导细胞凋亡,量子点的粒径大小为其引起细胞毒性的重要因素。
Objective To investigate the effects of quantum dots CdSe and CdTe with two different diameters on the proliferation, oxidative damage and apoptosis of L 929 cells. Methods MTT assay was used to detect the effects of three kinds of quantum dots on the proliferation of L 929 cells. The levels of malondialdehyde (MDA), superoxide dismutase (SOD) ), Glutathione peroxidase (GSH-Px) and hydroxyl radical (OH) content and apoptosis. Results IC50 values were 27.14 μg / mL for 2.2 nm CdSe, 28.28 μg / mL for 2 nm CdTe and 79.30 μg / mL for 3.5 nm CdTe at 24 h after exposure. Compared with 14 Cd / mL (P <0.05). The apoptotic rate of high dose group of three kinds of quantum dots was significantly different from that of the control group (P <0.05), and the difference of GSH-Px and .OH index was statistically significant Diameter CdTe, the differences between each dose group were statistically significant (P <0.05). Conclusion Quantum dots can inhibit the proliferation of mouse fibroblasts and induce oxidative damage and induce apoptosis. The size of quantum dots is an important factor in cytotoxicity.