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It has been known that there are at least three regulatory regions (NCR1, NCR2 and PCR) in the 5’-flanking sequence (from -610 bp to+1 bp) of human globin gene and that the function of PCR is unique to the human erythroleukemia (K562) cells. Here we have detected a DNA-binding protein factor (termed NFEa) in K562 cells,which can bind specifically to the PCR of human globin gene. The sequence of the binding site is 5’ACTGATG3’(between-222 bp and-216 bp). The NFEa is erythroidspecific and perhaps specific for K562 cells. It seemed that this factor differed from the erythroid-specific transcriptional factor (NFE-1) using competition assay. The presence of the NFEa further supported that the function of the cis-acting element PCR was specific for K562 cells, and helps us to understand the mechanism of the regulation of the expression of human globin gene in the human K562 cells.
It has been known that there are at least three regulatory regions (NCR1, NCR2 and PCR) in the 5’-flanking sequence (from -610 bp to + 1 bp) of human globin gene and that the function of PCR is unique to the Here we have detected a DNA-binding protein factor (termed NFEa) in K562 cells, which can bind specifically to the PCR of human globin gene. The sequence of the binding site is 5’ACTGATG3 ’(between -222 bp and-216 bp). The NFEa is erythroidspecific and perhaps specific for K562 cells. It seems that this factor differed from the erythroid-specific transcriptional factor (NFE-1) using competition assay. The presence of the NFEa further supported that the function of the cis-acting element PCR was specific for K562 cells, and helps us to understand the mechanism of the regulation of the expression of human globin gene in the human K562 cells.