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目的:研究惊天宁注射液中黄芩苷及芍药苷在大鼠体内的药动学,为临床用药提供参考。方法:惊天宁注射液静脉注射给予大鼠,不同时间点采血,甲醇沉淀法处理血浆样品,高效液相色谱方法测定黄芩苷及芍药苷血药浓度,并用DAS数据处理软件计算动力学参数。结果:惊天宁注射液静脉给药后,黄芩苷和芍药苷血药浓度-时间曲线在大鼠体内过程均符合二室模型,黄芩苷和芍药苷药时曲线下面积在实验剂量范围内符合线性药代动力学特征。结论:所建立的方法准确、灵敏度高,专属性好,可用于黄芩苷和芍药苷的体内血浆药物浓度的同时分析。血浆中黄芩苷及芍药苷消除较快。
OBJECTIVE: To study the pharmacokinetics of baicalin and paeoniflorin in rats in the preparation of santamanglin injection and to provide a reference for clinical use. Methods: The rats were injected with XTN and the plasma samples were collected by methanol precipitation method at different time points. The plasma concentrations of baicalin and paeoniflorin were determined by HPLC. The kinetic parameters were calculated by DAS data processing software. Results: The pharmacokinetics of baicalin and paeoniflorin in rats were all consistent with the two-compartment model. The area under the curve of baicalin and paeoniflorin was linear within the experimental dose range Pharmacokinetic characteristics. Conclusion: The established method is accurate, sensitive and specific. It can be used for the simultaneous analysis of plasma concentration of baicalin and paeoniflorin in vivo. Paeoniflorin and baicalin in plasma to eliminate faster.