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目的建立QuEChERS-超高效液相色谱-串联质谱法(UPLC-MS/MS),测定散装凉茶中6种非法添加的化学药物(对乙酰氨基酚、马来酸氯苯那敏、氢溴酸右美沙芬、安替比林、盐酸苯海拉明和萘普生等)。方法凉茶样品经含0.8%(V/V)甲酸的乙腈提取,C_(18)吸附剂净化,BEH C18超高效液相色谱柱(3.0 mm×150 mm,1.7μm)分离,多重反应监测(MRM)正离子模式扫描,标准曲线外标法定量。结果 6种化学药物的检出限为0.10μg/L~1.0μg/L;定量限为0.4μg/L~4.0μg/L;在线性范围内相关系数为0.998 4~0.999 4。3个不同添加水平下的平均加标回收率为72.3%~107.4%,RSD为1.2%~6.3%(n=6)。应用该方法对广州市售25份不同品牌散装凉茶样品进行测定,1份样品检出对乙酰氨基酚和马来酸氯苯那敏,浓度分别为721 mg/L和4.29 mg/L。结论该方法简便、快速、准确,可用于凉茶中非法添加的6种化学药物的快速筛查。
Objective To establish a QuEChERS-UPLC-MS / MS method for the determination of 6 kinds of illicitly added chemicals in bulk herbal tea (acetaminophen, chlorpheniramine maleate, hydrobromic acid right Methadone, antipyrine, diphenhydramine hydrochloride and naproxen, etc.). Methods Herbal tea samples were extracted with acetonitrile containing 0.8% formic acid (V / V), purified with C 18 adsorbent and separated on a BEH C18 column (3.0 mm × 150 mm, 1.7 μm). Multiple reaction monitoring ) Positive ion mode scanning, standard curve external standard method. Results The detection limits of the six chemicals ranged from 0.10μg / L to 1.0μg / L with the limits of quantitation of 0.4μg / L ~ 4.0μg / L. The correlation coefficients within the linear range were 0.998 4 ~ 0.999 4.3 different additions The average spike recoveries were 72.3% -107.4% with RSDs of 1.2% -6.3% (n = 6). Using this method, 25 samples of bulk herbal tea from different brands in Guangzhou were tested. One sample was detected with acetaminophen and chlorpheniramine maleate at concentrations of 721 mg / L and 4.29 mg / L, respectively. Conclusion The method is simple, rapid and accurate and can be used for the rapid screening of six kinds of chemicals illegally added in herbal tea.