论文部分内容阅读
目的探讨鼻咽癌细胞的细胞周期的生物节律和时辰照射对鼻咽癌细胞的作用。方法体外培养人鼻咽癌细胞CNE-2细胞系,在一天内不同的时间点(4AM、10AM、4PM、10PM)取出培养的细胞,用流式细胞仪检测细胞周期。根据细胞周期曲线一天内G2/M期的高峰和低谷分别照射鼻咽癌细胞,用微量细胞克隆形成法和平板克隆形成实验观察细胞存活率SF(survival fraction)。结果各细胞周期在4AM、10AM、4PM、10PM 4个时间点的分布,以单因素方差分析可见随时间变化差异有统计学意义(P<0.05)。微量细胞克隆形成法和平板克隆形成实验均显示细胞在一天内G2/M期的高峰和低谷分别照射,细胞存活率均有统计学意义(P<0.05),在高峰时照射的细胞SF小于低谷时照射。结论人鼻咽癌CNE-2细胞系的细胞周期随昼夜呈现节律性变化,时辰照射鼻咽癌细胞存活率的差异预示鼻咽癌时辰放疗的优越性。
Objective To investigate the biological rhythms of the cell cycle of nasopharyngeal carcinoma cells and the effect of time exposure on nasopharyngeal carcinoma cells. Methods Human nasopharyngeal carcinoma cell line CNE-2 was cultured in vitro. The cultured cells were removed at different time points (4 AM, 10 AM, 4PM and 10PM) in one day. The cell cycle was detected by flow cytometry. The nasopharyngeal carcinoma cells were irradiated with the G2 / M peak and the trough according to the cell cycle curve. The survival fraction (SF) of the cells was observed by microcolony formation assay and plate clone formation assay. Results The distribution of each cell cycle at 4 AM, 10 AM, 4 PM and 10 PM at 4 time points showed that the difference with time was statistically significant (P <0.05) by single factor analysis of variance. Micro-cell cloning and plate clone formation assay showed that the cells were irradiated at the peak and the trough of G2 / M phase in one day, respectively. The cell survival rates were all statistically significant (P <0.05) When the irradiation. Conclusions The cell cycle of human nasopharyngeal carcinoma CNE-2 cell line shows rhythmic changes with the diurnal variation. The difference of the survival rate of nasopharyngeal carcinoma cells at the same time indicates the superiority of time radiotherapy in nasopharyngeal carcinoma.