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目的观察加巴喷丁(GBP)灌胃对小鼠镇痛、自主活动和学习记忆的影响。方法按分层随机区组设计,将200小鼠分为甩尾法、扭体法、镇静实验、避暗法和跳台法实验组,每实验组40只小鼠,再分为4小组(n=10):生理盐水组(NS组),3个剂量(50,100,200 mg·kg-1)GBP组即GBP50、GBP100、GBP200组。给药一次后,测试小鼠甩尾潜伏期、15 min内扭体次数、5 min内自主活动次数、避暗潜伏期及错误次数、跳台潜伏期及错误次数。结果在甩尾法和扭体法实验中,GBP可产生镇痛作用(P<0.05或P<0.01);自主活动实验中,G100、G200组小鼠表现有镇静作用(P<0.01);在避暗实验和跳台实验中,G100、G200组的潜伏期缩短,错误次数增加(P<0.05)。结论加巴喷丁灌胃产生了镇痛、镇静和遗忘作用。
Objective To observe the effects of gabapentin (GABA) on analgesia, autonomic activity and learning and memory in mice. Methods According to the stratified randomized block design, 200 mice were divided into tail flick method, writhing method, sedation experiment, avoidance method and jumping test method. Each experimental group was divided into 4 groups (n = 10): saline group (NS group), three doses (50,100,200 mg · kg-1) of GBP group GBP50, GBP100, GBP200 group. After administration for one time, mice were tested for tail flick latency, number of writhing within 15 min, number of autonomous activities within 5 min, latency to avoid darkness and errors, jump latency and number of errors. Results GBP produced analgesic effects (P <0.05 or P <0.01) in the tail flick method and writhing test. In the spontaneous activity test, G100 and G200 mice sedated (P <0.01) In the dark-avoidance experiment and the platform-jump experiment, the latency of G100 and G200 groups was shortened and the number of errors was increased (P <0.05). Conclusion Gabapentin has analgesic, sedation and amnesia effects.