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探讨北太平洋鱿鱼墨多糖与秘鲁鱿鱼墨多糖对化疗模型小鼠肠道菌群的影响。将40只Balb/c小鼠随机分为4组:正常组、模型组、北太平洋鱿鱼墨多糖组、秘鲁鱿鱼墨多糖组。采用环磷酰胺化疗模型,无菌收集小鼠粪便并提取细菌基因组DNA,通过构建各组小鼠肠道细菌的16S rDNA克隆文库,单酶切分型,观察鱿鱼墨多糖对肠道菌群的作用。试验结果表明:两种鱿鱼墨多糖对化疗模型小鼠肠道菌群种类有一定调节作用,且两种鱿鱼墨多糖效果不尽相同。
To investigate the influence of North Pacific squid polysaccharides and Peru squid ink polysaccharides on the intestinal flora of mice with chemotherapy. Forty Balb / c mice were randomly divided into four groups: normal group, model group, North Pacific squid polysaccharide group and Peru squid ink polysaccharide group. Using cyclophosphamide chemotherapy model, the mice feces was collected aseptically and the bacterial genomic DNA was extracted. By constructing the 16S rDNA clone library of intestinal bacteria in each group and digesting them by single enzyme digestion, the effects of squid polysaccharides on intestinal flora effect. The results showed that: two kinds of squid ink polysaccharides on the model of intestinal flora in mice have a moderating effect, and the two squid ink polysaccharides vary.