论文部分内容阅读
我们建立了兔骨膜成骨细胞的体外培养方法,并借鉴国内外近年报道的成骨细胞定性方法,在形态学、组织化学及生物特性测定等方面做了研究,证实该培养细胞具有成骨细胞的典型特征:1活细胞光镜下形态的相符性;2电镜超微结构观察有凹陷的细胞核、胞浆内含有大量的线粒体、扩张的粗面内质网及分泌空泡并富含电子致密物;3高的碱性磷酸酶活性;4在体外具有一定的特异性的钙化能力。该培养物可作为体外研究骨代谢的生物学模型,也为活体种植成骨的研究提供了资料。
We established a rabbit periosteum osteoblast in vitro culture methods, and draw on the qualitative methods of osteoblasts reported in recent years at home and abroad, in morphology, histochemistry and biological characteristics of the determination of a study to confirm that the cultured cells with osteoblasts The typical characteristics of a living cell: Morphology of living cells under light microscopy; 2 ultrastructure of electron microscopy concave nuclei, the cytoplasm contains a large number of mitochondria, expansion of the rough endoplasmic reticulum and the secretion of vacuolar and electron-dense 3 high alkaline phosphatase activity; 4 in vitro with a certain degree of specific calcification. The culture can be used as a biological model for the study of bone metabolism in vitro and also provide information for the study of osteogenesis in vivo.