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目的检测微小RNA-21(MicroRNA-21)和程序性细胞死亡因子4(PDCD4)在非小细胞肺癌(NSCLC)中的表达情况,分析两者之间的相关性并探讨其临床意义。方法采用实时荧光定量PCR(qRT-PCR)、原位杂交和免疫组化方法检测MicroRNA-21和PDCD4在癌旁正常肺组织、非典型增生组织、鳞癌组织和腺癌组织中的表达情况。结果MicroRNA-21在肺鳞癌和腺癌组织中的表达高于癌旁正常肺组织及非典型增生肺组织(P<0.05);PDCD4mRNA及蛋白在肺鳞癌和腺癌组织中的表达均低于癌旁正常肺组织及非典型增生组织(P<0.05),非典型增生组织与癌组织之间的差异有统计学意义(P<0.05);肺腺癌与鳞癌之间的差异无统计学意义(P>0.05)。免疫组化及原位杂交检测PDCD4蛋白和PDCD4mRNA具有一致性(r=0.512,P<0.01);在NSCLC中MicroRNA-21与PDCD4mRNA的表达呈负相关性(r=-0.910,P<0.01),MicroRNA-21与PDCD4蛋白的表达呈负相关(r=-0.876,P<0.01)。结论 MicroRNA-21与PDCD4在NSCLC中的表达呈负相关;MicroRNA-21/PDCD4环路异常促进了NSCLC的发生发展。
Objective To detect the expression of microRNA-21 (MicroRNA-21) and programmed cell death factor 4 (PDCD4) in non-small cell lung cancer (NSCLC), and to analyze the correlation between the two and explore its clinical significance. Methods Real-time quantitative PCR (qRT-PCR), in situ hybridization and immunohistochemistry were used to detect the expression of MicroRNA-21 and PDCD4 in normal lung tissues, atypical hyperplasia tissues, squamous cell carcinoma and adenocarcinoma tissues. Results The expression of MicroRNA-21 in squamous cell carcinoma and adenocarcinoma tissues was higher than that in adjacent normal lung tissues and atypical hyperplastic lung tissues (P <0.05). The expression of PDCD4 mRNA and protein in lung squamous cell carcinoma and adenocarcinoma tissues were both lower There were significant differences between normal lung tissue and atypical hyperplasia (P <0.05) and between atypical hyperplasia and carcinoma (P <0.05). There was no statistical difference between lung adenocarcinoma and squamous cell carcinoma Significance (P> 0.05). The expression of PDCD4 protein and PDCD4 mRNA were consistent (r = 0.512, P <0.01) by immunohistochemistry and in situ hybridization. There was a negative correlation between the expression of MicroRNA-21 and PDCD4 mRNA in NSCLC (r = -0.910, MicroRNA-21 and PDCD4 protein expression was negatively correlated (r = -0.876, P <0.01). Conclusion The expression of MicroRNA-21 and PDCD4 in NSCLC is negatively correlated. The abnormality of MicroRNA-21 / PDCD4 loop promotes the development of NSCLC.