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目的:观察sPD-1蛋白对小鼠H22肝癌腹水瘤生长的抑制作用。方法:建立H22肝癌小鼠腹水瘤模型,将24只接种H22肝癌细胞BALB/c小鼠随机分成3组,其腹腔分别注射PBS(模型组)、DDP(阳性对照组)以及sPD-1蛋白,用药15 d后,停止用药,取腹水测定腹水细胞Treg细胞的产生情况,并观察小鼠的毒副反应以及生存期。结果:sPD-1蛋白组的H22肝癌生命延长为(25.75±4.30)d,DDP组的H22肝癌生命延长为(25.00±4.41)d,均明显高于对照组的(16.63±2.67)d(P<0.05)。sPD-1蛋白组小鼠腹水中Treg细胞明显少于DDP组和模型组,免疫水平优于DDP和PBS组。结论:原核表达的sPD-1蛋白对小鼠H22肝癌腹水瘤的生长具有明显抑制作用,并能促进机体免疫系统功能,提高生存期。
Objective: To observe the inhibitory effect of sPD-1 protein on the growth of mouse H22 hepatocellular carcinoma ascites tumor. Methods: H22 hepatocellular carcinoma mouse model of ascites tumor was established. Twenty-four H22 hepatocellular carcinoma BALB / c mice were randomly divided into three groups: PBS (model group), DDP (positive control group) and sPD- After 15 days of treatment, the drug was stopped and the ascites was used to measure the Treg cells. The toxicity and survival of mice were observed. Results: The lifespan of H22 hepatocarcinoma in sPD-1 group was (25.75 ± 4.30) d and that in DDP group was (25.00 ± 4.41) d, which were significantly higher than that in control group (16.63 ± 2.67) d <0.05). Treg cells in ascites of sPD-1 protein group were significantly less than that of DDP group and model group, and the immunological level was better than that of DDP group and PBS group. CONCLUSION: The prokaryotic expression of sPD-1 protein has a significant inhibitory effect on the growth of mouse H22 hepatocellular carcinoma ascites tumor and can promote the body’s immune system function and improve survival.