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目的建立酒精诱导的氧化应激小鼠模型,对姜黄素的抗氧化活性进行初步评价,探讨补充生物活性因子的抗氧化功能评价方法。方法8周龄,昆明小鼠60只,动物房适应1周,按体重随机分为5组。实验周期内,每日按0.2ml/10g·BW给予各组实验小鼠连续灌服相应剂量受试物。实验结束时,处死全部小鼠,取血,测定肝脏相关生化指标。结果酒精模型组血浆和肝脏谷胱甘肽过氧化酶GPx的酶活性均显著低于正常对照组(P<0.05);肝脏蛋白质羰基含量(PCO)显著高于正常对照组(P<0.05)。结论长期摄入低浓度酒精,可以诱导建立氧化应激小鼠模型;实验中姜黄素的抗氧化活性得到初步证实;蛋白质羰基含量的测定显示,其可有效补充生物活性因子抗氧化功能评价方法。
OBJECTIVE: To establish an alcohol-induced oxidative stress mouse model to evaluate the antioxidant activity of curcumin and to evaluate the antioxidant activity of the bioactive factor. Methods Eight-week-old Kunming mice (60 animals) were housed for one week and divided into five groups according to body weight. During the experimental period, the mice in each group were given 0.2 ml / 10 g · BW daily for continuous administration of the corresponding doses of the test substance. At the end of the experiment, all mice were sacrificed and blood was collected for the determination of liver-related biochemical markers. Results The enzyme activities of GPx in plasma and liver of alcohol model group were significantly lower than those of normal control group (P <0.05). The content of carbonyl group in liver protein (PCO) was significantly higher than that of normal control group (P <0.05). Conclusion Long-term intake of low concentration of alcohol can induce the establishment of oxidative stress mouse model. The antioxidant activity of curcumin was initially confirmed in the experiment. The determination of carbonyl content of the protein showed that it could effectively supplement the antioxidant activity of bioactive factor.