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1 实验材料 采用wister大鼠,体重260±28G,雌雄兼用,购于军事医学科学院卫生环境医学研究所动物室。高良姜水提物:用水煎醇沉法制备成150％浓度,冷藏备用。丹参水提物:制法、浓度同上。肝素:常州生物化学制药厂生产。批号:930622。异丙肾上腺素(ISO):上海天丰制药厂生产。批号:911103。生理盐水(0.9w/v％)。2 实验方法 体外抗凝血实验:取玻璃试管30支,分为5组,每组6支,均帖上标记。盐水组:试管内注入0.9％生理盐水0.15ml。丹参组:注入150％浓度丹参水提物0.15ml。肝素组:
1 Experimental materials Wister rats weighing 260 ± 28G, both male and female, were purchased from the Animal Laboratory of the Institute of Hygiene and Environmental Medicine, Academy of Military Medical Sciences. Galangal water extract: prepared by water decoction and alcohol precipitation at a concentration of 150% and frozen for use. Salvia miltiorrhiza extract: The preparation method and concentration are the same as above. Heparin: Produced by Changzhou Biochemical Pharmaceutical Factory. Lot number: 930622. Isoprenaline (ISO): manufactured by Shanghai Tianfeng Pharmaceutical Factory. Lot number: 911103. Normal saline (0.9w/v%). 2 Experimental methods In vitro anticoagulant experiments: Take 30 glass test tubes, divided into 5 groups, 6 in each group, all marked on the posts. Saline group: 0.15 ml of 0.9% normal saline was injected into the tube. Salvia miltiorrhiza group: Infusion of 0.15 ml of 150% Salvia miltiorrhiza water extract. Heparin group: