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目的:研究固本防哮饮对哮喘缓解期小鼠肺组织白介素4(IL-4)、白介素12(IL-12)的干预作用,探讨其治疗哮喘的免疫学机制。方法:75只小鼠随机分为正常组、模型组、预防组、固本防哮饮组和孟鲁司特钠组,以卵蛋白(OVA)和呼吸道合胞病毒(RSV)联合致敏激发制备哮喘缓解期小鼠模型。观察各组小鼠的一般行为活动和病理学变化特点,用RT-PCR法测定哮喘小鼠肺组织IL-4、IL-12的m RNA表达。结果:模型组小鼠肺组织可见支气管壁水肿、增厚,周围有嗜酸性粒细胞和中性粒细胞浸润。预防组、固本防哮饮组及孟鲁司特钠组,小鼠肺病理改变显著改善,支气管壁充血水肿现象减轻,管壁和平滑肌厚度减小,且嗜酸性粒细胞和中性粒细胞浸润也显著减少。模型组小鼠肺组织IL-4 m RNA较正常组显著高表达(P<0.05),而IL-12 m RNA较正常组低表达(P<0.05)。预防组、固本防哮饮组、孟鲁司特钠组IL-4 m RNA较模型组表达下降(P<0.05),而IL-12 m RNA较模型组表达升高(P<0.05)。结论:固本防哮饮防治哮喘的机制可能是通过降低IL-4 m RNA同时升高IL-12 m RNA表达来纠正Th1/Th2失衡,从而减轻气道炎性反应,缓解气道重塑。
Objective: To study the intervention effect of Guben anti-asthmatic beverage on interleukin-4 (IL-4) and interleukin-12 (IL-12) in the lung tissue of asthmatic mice during remission period and to explore its immunological mechanism of treating asthma. Methods: Seventy-five mice were randomly divided into normal group, model group, prophylaxis group, Guben anti-asthma drink group and montelukast sodium group, and sensitized with OVA and RSV Preparation of asthma remission mouse model. The general behavior and pathological changes of mice in each group were observed. The mRNA expression of IL-4 and IL-12 in the lung tissue of asthmatic mice was detected by RT-PCR. Results: The bronchial wall edema and thickened in the lung tissue of mice in model group with infiltration of eosinophils and neutrophils around. Prevention group, Guben anti-asthma drink group and montelukast sodium group, lung pathological changes significantly improved in mice, bronchial wall edema eased phenomenon, wall and smooth muscle thickness decreased, and eosinophils and neutrophils Infiltration is also significantly reduced. IL-4 mRNA expression in lung tissue of model group was significantly higher than that of normal group (P <0.05), while IL-12 mRNA was lower than that of normal group (P <0.05). The levels of IL-4mRNA in prevention group, Guben anti-asthma drink group and montelukast sodium group were lower than those in model group (P <0.05), while the expression of IL-12 mRNA in model group was higher than that in model group (P <0.05). Conclusion: The mechanism of Guben anti-asthma drink in preventing and treating asthma may be to correct the imbalance of Th1 / Th2 by decreasing IL-4 m RNA and IL-12 m RNA expression, thereby reducing airway inflammatory reaction and relieving airway remodeling.