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目的 :为了进一步了解间歇性气囊挤压法 ( Intermittent pneumatic compression,IPC)挤压大鼠腿部与一氧化氮 ( NO)的关系。方法 :检测了大鼠骨骼肌中 3种一氧化氮合酶 ( NOS)同工酶 :神经型 NOS( n NOS) ;诱导型 NOS( i NOS)和内皮细胞型 NOS( e NOS) m RNA在 IPC作用后的表达变化。 2 5只 SD大鼠被随机分为 3个模拟实验组和 4个 IPC实验组。每只鼠取右侧胫前肌( AT)和提睾肌 ( CM)作为正常对照。 IPC组挤压 0 .5 ,1,和 5 h,及挤压 5 h加等待 4h,模拟实验组除不挤压外 ,其他操作均与实验组相同 ,然后分离左侧 AT和 CM作为处理后样品。所有样品应用 RT-PCR进行 NOS m RNA测定。以样品中看家基因 2 ,3 -二羟基丙醛 -3 -磷酸脱氢酶 ( GAPHD) c DNA为内参 ,与 NOS c DNA共同扩增。 PCR产物电泳条带密度用 NIH图像分析软件定量 ,并以与正常对照的对比值作为变化比率。结果 :在 IPC作用 0 .5、1和 5 h后 ,e NOSm RNA显著上升 ,在 AT中分别达到正常对照的 1.2 ,1.8和2 .6倍 ;在 CM中分别达到 1.2 ,1.8和 2 .7倍 ,而其他 NOS,除 5 h IPC组的 n NOS外 ,总体表现下调。在 IPC作用 1h加等待 4h组中 ,e NOS m RNA回复至正常对照水平。结论 :该结果证实了 IPC产生的机械压力至少部分增加了血管壁的剪切压 ,使内皮细?
OBJECTIVE: To further understand the relationship between the leg and nitric oxide (NO) in rats with Intermittent Pneumatic Compression (IPC). Methods: Three kinds of nitric oxide synthase (NOS) isoenzymes: NOS (nNOS), inducible NOS (iNOS) and endothelial NOS (eNOS) m RNA in rat skeletal muscle Changes after IPC. Twenty-five SD rats were randomly divided into three simulated experimental groups and four IPC experimental groups. Right mouse anterior tibialis (AT) and cremasteric muscle (CM) were taken as normal control. IPC group extrusion 0.5, 1, and 5 h, and squeeze 5 h plus wait 4 h, the simulated experimental group except not squeezed, the other operations were the same as the experimental group, and then the left AT and CM were isolated after treatment sample. All samples were assayed for NOS m RNA using RT-PCR. In this study, housekeeping gene 2, 3 - dihydroxy - propionaldehyde - 3 - phosphate dehydrogenase (GAPHD) c DNA was used as an internal reference to amplify with NOS c DNA. The electrophoretic banding density of PCR products was quantified using NIH image analysis software and the ratio of change to normal controls was used. RESULTS: eNOSm RNA increased significantly at 1.2, 1.8, and 2.6 times of normal control at AT, respectively, after IP exposure for 0.5, 1, and 5 h, reaching 1.2, 1.8 and 2.7 in CM, respectively Fold, while other NOS, except for n-NOS in 5 h IPC group, showed an overall decrease. The eNOS m RNA returned to the normal control level in 1h IPC plus 4h IPC. CONCLUSIONS: This result demonstrates that the mechanical pressure generated by IPC at least partially increases the shear pressure on the vessel wall, rendering the endothelium thinner.