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目的 研究无症状人免疫缺陷病毒 (HIV)感染者和艾滋病患者外周血中自然杀伤(NK)细胞亚群在白细胞分化抗原 (CD) 3 - 淋巴细胞所占百分比的变化状况。方法 用流式细胞技术检测三色荧光抗体标记的 2 3例HIV感染者及 8名健康人外周血中各类NK亚群细胞表面抗原的表达和CD4 + T淋巴细胞绝对计数。结果 无症状HIV感染者和艾滋病患者外周血中的CD5 6 + CD16 +CD3- NK细胞亚群百分比 (均值分别为 2 4 69% ,2 4 90 % )均低于健康人 (均值 3 9 99% ,P <0 0 5) ;无症状HIV感染者CD5 6 - CD16 + CD3 - NK细胞亚群百分比 (均值 12 58% )明显高于健康对照组 (均值 6 51% ,P <0 0 1) ;艾滋病患者外周血中的CD5 6- CD16+ CD3- NK细胞亚群百分比 (均值15 47% )明显高于健康人 (均值 6 51% ,P <0 0 5) ;艾滋病患者的CD5 6 + CD16 - CD3 - NK细胞亚群百分比 (均值 3 50 % )明显低于无症状HIV感染者 (均值 6 75% ,P <0 0 5)。结论 HIV感染使NK细胞不同亚群在外周血中百分比发生变化 ,可能与艾滋病疾病进展相关
Objective To study the changes of the percentage of natural killer (NK) cell subsets in the peripheral blood of asymptomatic HIV / AIDS patients and AIDS patients in the percentage of leukocyte differentiation antigen (CD) 3 - lymphocytes. Methods Flow cytometry was used to detect the cell surface antigens and the absolute counts of CD4 + T lymphocytes in peripheral blood of 23 HIV-infected patients and 8 healthy volunteers labeled with tri-color fluorescent antibody. Results The percentages of CD56 + CD16 + CD3 + NK cell subsets in peripheral blood of asymptomatic HIV infected persons and AIDS patients (mean 24.4% and 24.4% respectively) were lower than those in healthy people (mean, 39 9% , P <0.05). The percentages of CD5 6 - CD16 + CD3 - NK cells in asymptomatic HIV infected individuals (mean 12 58%) were significantly higher than those in healthy controls (mean 6 51%, P 0 01). The percentage of CD5 6 - CD16 + CD3 - NK cell subsets (15 47%) in peripheral blood of AIDS patients was significantly higher than that of healthy individuals (mean 6 51%, P 0 05). The percentage of CD5 6 + CD16 - CD3 - The percentage of NK cell subsets (mean 3 50%) was significantly lower than those of asymptomatic HIV infected individuals (mean 6 75%, P 0 05). Conclusions HIV infection may change the percentage of NK cell subsets in peripheral blood and may be related to the progression of AIDS