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本组实验研究采用萤光素评定皮瓣动脉化后活力的方法在动物模型中用作评定肠管活力,并确定此指示剂的安全、可靠和准确性。资料和方法:萤光素是一种有机染料,当暴露在3600～4000A(埃氏单位)紫外光线下时,发出金绿色萤光。静脉注射该染料后,迅速在细胞外液中出现,且只进入有活力的细胞内,几分钟内可在组织中达到最高浓度,以后在24～36小时内有规则地从泌尿道排尽。注射萤光素后的病人,机体所有组织均被染色,致皮肤和尿均成深黄色。通过食物或药物摄取萤光素
This group of experimental studies using fluorescein flap method of assessing the vitality of the flap in animal models for assessing intestinal viability and to determine the safety, reliability and accuracy of this indicator. Materials and Methods: Luciferin is an organic dye that emits gold-green fluorescence when exposed to ultraviolet light in the range of 3600-4000 A (units of Ellison). Intravenous injection of the dye, the rapid emergence in the extracellular fluid, and only enter the viable cells, within minutes to reach the highest concentration in the tissue, after 24 to 36 hours regularly from the urinary tract exhaust. After injection of luciferin patients, all tissues are stained, causing the skin and urine are dark yellow. Ingesting luciferin through food or medicine