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目的研究花色苷(Anth)体外抗氧化及改善胰岛素抗性(IR)的效应,并分析构效关系。方法以自然界最为常见的6种Anth作为受试物,测定其对二苯代苦味酰肼(1-diphenyl-2-picrylhydrazyl,DPPH?)自由基的清除能力,及其对H2O2诱导的IR3T3-L1脂肪细胞胞内活性氧(reactive oxygen species,ROS)水平和葡萄糖摄取能力的影响。结果6种Anth均具有较强的DPPH自由基清除能力,效应和B环上酚羟基数目有关;6种Anth中,具有邻苯二酚结构的2种能更好的清除H2O2诱导IR脂肪细胞胞内的ROS,同时显著提高胰岛素刺激后脂肪细胞对葡萄糖的摄取能力,呈剂量-效应依赖型。结论花色苷可预防和改善氧化应激引起的3T3-L1脂肪细胞胰岛素抗性,此效应与B环上的邻苯二酚结构有关。
Objective To study the effects of anthocyanin on anti-oxidation and insulin resistance (IR) in vitro and to analyze the structure-activity relationship. Methods Six kinds of anthocyanins, the most common in nature, were used as test substances to determine their ability of scavenging free radicals of 1-diphenyl-2-picrylhydrazyl (DPPH?) And its effect on H2O2-induced IR3T3-L1 Effects of intracellular reactive oxygen species (ROS) levels and glucose uptake capacity in adipocytes. Results All six anthocyanins had strong DPPH radical scavenging activity, which was related to the number of phenolic hydroxyl groups on the B ring. Two of the six anthocyanins with catechol structure could better eliminate H2O2-induced IR adipocyte ROS, while significantly increasing insulin-stimulated adipocyte glucose uptake capacity, was dose-effect-dependent. Conclusion Anthocyanins can prevent and improve the insulin resistance induced by oxidative stress in 3T3-L1 adipocytes, which is related to the structure of catechol on B ring.