论文部分内容阅读
目的:在细胞水平,从Neu-p12、Neu-p68、Neu-p150中筛选可以改善胰岛素抵抗的药物。方法:采用“鸡尾酒”法,诱导分化出成熟的3T3-L1脂肪细胞,300μM油酸(OA)作用6 h建立胰岛素抵抗的细胞模型。实验分为5组,对照组、10 nM褪黑素组、10 nM Neu-p12组、10 nM Neu-p68组和10 nM Neu-p150组,采用2-脱氧-[3H]-D-葡萄糖的摄取实验,测定进入细胞内的葡萄糖的差异。结果:加药组的葡萄糖摄取率分别增加了508%、740%、499%、316%。结论:10 nM Neu-p12、10 nM Neu-p68、10 nM Neu-p150都可以促进胰岛素抵抗的3T3-L1脂肪细胞对葡萄糖的吸收,并且10 nM Neu-p12的效果最好。
OBJECTIVE: To screen for drugs that can improve insulin resistance at the cellular level from Neu-p12, Neu-p68 and Neu-p150. Methods: The mature 3T3-L1 adipocytes were induced by “Cocktail” method, and a cell model of insulin resistance was established by using 300μM oleic acid (OA) for 6 hours. The experiment was divided into 5 groups, control group, 10 nM melatonin group, 10 nM Neu-p12 group, 10 nM Neu-p68 group and 10 nM Neu-p150 group, using 2-deoxy- [ Ingestion experiments determine the difference in glucose entering the cells. Results: The glucose uptake rate in the dosing group increased by 508%, 740%, 499% and 316% respectively. CONCLUSION: 10 nM Neu-p12, 10 nM Neu-p68 and 10 nM Neu-p150 all promote glucose uptake in insulin-resistant 3T3-L1 adipocytes and 10 nM Neu-p12 works best.