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目的了解植物化学物芒果苷亚急性毒性作用,确保芒果苷食用安全性。方法成熟健康的清洁级Wistar大鼠80只随机分为低、中、高剂量芒果苷组(2.75、5.50、11.00 g/kg)和对照组,每组雌雄各10只,连续喂养30 d,观察大鼠一般状态、称量体重并计算食物利用率;采用全自动生化分析仪检测血液学及血液生化学各项指标;观察主要脏器外观及病理学改变。结果对照组及芒果苷各剂量组大鼠生长发育状态良好、饮食饮水正常,活动自如,无死亡;雄性对照组、低、中、高剂量芒果苷组大鼠体重分别为(280.6±18.82)、(279.3±15.55)、(279.8±11.73)、(276.7±19.94)g,体重增长分别为(189.5±17.90)、(188.7±16.69)、(189.2±11.73)、(185.9±17.55)g,食物利用率分别为(28.1±3.07)、(28.6±2.37)、(27.9±0.60)、(27.3±1.92)%;雌性对照组、低、中、高剂量芒果苷组大鼠体重分别为(202.4±12.26)、(204.6±17.20)、(202.1±15.12)、(201.3±12.30)g,体重增长分别为(120.5±14.50)、(121.9±17.50)、(120.2±18.24)、(121.5±15.70)g,食物利用率分别为(22.4±2.60)、(20.5±8.76)、(20.9±2.14)、(21.8±1.64)%;与对照组比较,各剂量芒果苷组大鼠体重、体重增重及食物利用率无明显变化;与对照组比较,各剂量芒果苷组大鼠血常规、血液生化学等指标及各主要脏器系数无明显变化;高剂量芒果苷组大鼠肝、脾、肾、睾丸、卵巢组织结构未见病理性改变。结论以11.00 g/kg剂量芒果苷喂饲大鼠30 d,对大鼠生长发育及主要脏器无毒性作用。
Objective To understand the subacute toxicity of phytochemicals mangiferin to ensure the safety of mangiferin. Methods Totally 80 healthy and clean Wistar rats were randomly divided into low, medium and high doses of mangiferin (2.75,5.50,11.00 g / kg) and control group, with 10 in each group, fed continuously for 30 days. The general state of rats was weighed and body weight was calculated and food utilization rate was calculated. The indexes of hematology and blood biochemistry were detected by automatic biochemical analyzer. The appearances and pathological changes of major organs were observed. Results The rats in control group and mangiferin groups had good growth and development status, normal drinking water and no death. The body weight of male control group, low, medium and high dose mangiferin groups were (280.6 ± 18.82), (279.3 ± 15.55), (279.8 ± 11.73) and (276.7 ± 19.94) g respectively, the body weight gain were (189.5 ± 17.90), (188.7 ± 16.69), (189.2 ± 11.73) and (28.1 ± 3.07), (28.6 ± 2.37), (27.9 ± 0.60) and (27.3 ± 1.92)%, respectively. The body weight of female control group, low, medium and high dose mangiferin groups were (202.4 ± 12.26 (120.5 ± 14.50), (121.9 ± 17.50), (120.2 ± 18.24), (121.5 ± 15.70) g, (204.6 ± 17.20), (202.1 ± 15.12) and The food utilization rates were (22.4 ± 2.60), (20.5 ± 8.76), (20.9 ± 2.14) and (21.8 ± 1.64)%, respectively. Compared with the control group, body weight, body weight gain and food use Compared with the control group, there was no significant change in blood routine, blood biochemistry and other major organ coefficients of rats in each dose of mangiferin group. The liver, spleen, kidney Testicular, ovarian tissue structure and no pathological changes. Conclusion Mangiferin at a dosage of 11.00 g / kg was administered to rats for 30 days, which had no toxic effect on the growth and development of rats and the main organs.