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基于近红外光谱法对淫羊藿中有效成分淫羊藿苷和朝藿定C的含量进行快速同时定量分析,采用偏最小二乘法(PLS)建立近红外光谱信息与预测组分含量间的数学校正模型。结果表明通过标准正态变量转换(SNV)与一阶导数对光谱进行预处理,所建立的淫羊藿苷和朝藿定C的校正模型效果最佳。淫羊藿苷与朝藿定C校正集均方差(RM SEC)分别为0.217、0.718;相关系数分别为0.9825、0.9863;预测均方差(RM SEP)分别为0.155,0.297。该方法简单、准确,更适合于淫羊藿药材中主要成分的快速分析。
Based on the rapid and simultaneous quantitative analysis of the contents of icariin and epimedin C in epimedium by near infrared spectroscopy, the mathematical method of near-infrared spectral information and prediction of the content of the components was established by partial least squares (PLS) Correction model. The results showed that the calibration model of icariin and epimedin C was the best by pretreating the spectra by standard normal variable transformation (SNV) and first derivative. The corrected mean square error (RM SEC) of icariin and epimedin C were 0.217 and 0.718, respectively, and the correlation coefficients were 0.9825 and 0.9863 respectively. The mean square error of prediction (RM SEP) was 0.155 and 0.227 respectively. The method is simple and accurate, and is more suitable for rapid analysis of the main components of Epimedium.