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目的探讨大鼠后肢缺血再灌注(I/R)后肾组织核因子-κB(NF-κB)的变化及血必净对骨骼肌I/R后肾损伤的保护作用。方法雄性SD大鼠制作I/R模型,随机分为3组:A组为对照组,仅进行常规麻醉,不阻断后肢血流;B组为缺血组,即缺血4h无再灌注组;C组为I/R组,即缺血4h后再灌注6h;D组为I/R+血必净低剂量组,即缺血4h再灌注6h,并于缺血前10min及再灌注开始时即刻分别尾静脉注射血必净8mL/kg;E组为I/R+血必净高剂量组,即缺血4h再灌注6h并于缺血前10min及再灌注开始时即刻分别尾静脉注射血必净16mL/kg。检测血清中肌酸激酶(CK)含量,肾组织中MDA含量,NF-κB基因在肾组织中的表达。结果肾组织中NF-κB表达A组不明显;在B,C组呈上升趋势;D,E组表达下调;其余4组与A组比较,差异均有统计学意义(P<0.01);D,E两组与C组比较,差异有统计学意义(P<0.01);而D,E两组差异无统计学意义(P>0.05)。CK和MDA含量:B,C组逐渐升高,C组较B组升高明显(P<0.01);药物治疗后的D,E组相应下降,与C组比较,差异均有统计学意义(P<0.01)。结论在I/R后肾组织中NF-κB的表达明显上调,血必净可抑制NF-κB表达上调,对I/R肾损伤有明显保护作用,此作用与本实验设计的剂量无关。
Objective To investigate the changes of nuclear factor-κB (NF-κB) in the rat hind limbs after ischemia-reperfusion (I / R) and the protective effect of Xuebijing on skeletal muscle I / R injury. Methods Male Sprague-Dawley rats were randomly divided into 3 groups: group A (control group), conventional anesthesia alone, and no blockade of blood flow in the hind limbs. Group B was ischemic, ie, ischemia-reperfusion group ; Group C was I / R group, which was reperfused for 6h after 4h ischemia; group D was I / R + Xuebijing low dose group, which was reperfused for 6h after 4h of ischemia and 10min before ischemia and at the beginning of reperfusion Immediate intravenous injection of blood sodium chloride 8mL / kg; E group I / R + Xuebijing high-dose group, ischemia and reperfusion 4h 4h and 10min before ischemia and immediately after reperfusion, respectively, tail vein injection of blood will be Net 16mL / kg. Serum creatine kinase (CK) content, MDA content in renal tissue and NF-κB gene expression in renal tissue were detected. Results The expression of NF-κB in renal tissue was not obvious in group A, increased in groups B and C, and decreased in groups D and E. The differences between the other 4 groups and group A were statistically significant (P <0.01) (P <0.01). There was no significant difference between the two groups (P> 0.05). The levels of CK and MDA in group B and C increased gradually, while those in group C were significantly higher than those in group B (P <0.01), while those in group D and E decreased after treatment. The difference was statistically significant (P < P <0.01). Conclusion The expression of NF-κB in I / R renal tissues is significantly up-regulated. Xuebijing can inhibit the up-regulation of NF-κB and protect I / R renal injury. This effect has nothing to do with the designed dose.