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目的对脐血进行系列研究,以期为临床应用提供实验依据。方法采用分离新鲜脐血单个核细胞,未行或行低剂量60Coγ射线62 mCy照射,并用3H-TdR掺入和释放法、ELISA分析、流式细胞仪、MTT和粒巨噬细胞集落形成单位测定等方法对脐血量、单个核细胞(MNC)数量、活性MNC百分率、CD34+细胞数及其与新生儿月龄、性别、脐血血象及产妇年龄关系、脐血T淋巴细胞、NK细胞活性、低剂量照射脐血后T淋巴细胞和NK细胞活性变化、脐血清集落刺激活性物质和临床用于白细胞减少症疗效进行了实验和临床研究。结果每份脐血在100~150 ml范围内占95.4%,其量与MNC无相关性,存放在6℃冰箱3 d以内较适宜,活性MNC在91.2%以上;脐血中CD34+细胞数除与性别相关外,与其他因素无相关性;脐血T淋巴细胞和NK细胞活性偏低,低剂量照射能提高其活性;脐血清富含细胞集落刺激活性物质,以IL-3为主;白细胞减少症患者输注脐血可提升外周血白细胞总数水平。结论由于以上诸多优势,故脐带血具有临床应用的广阔前景。
Objective To study cord blood in order to provide experimental evidence for clinical application. Methods Fresh umbilical cord blood mononuclear cells were isolated and irradiated with low-dose 60Coγ-rays at a dose of 62 mCy. The 3H-TdR incorporation and release assay, ELISA, flow cytometry, MTT and granulocyte colony forming units The quantity of cord blood, the number of mononuclear cells (MNC), the percentage of active MNCs, the number of CD34 + cells and their relationship with the age, sex, umbilical cord blood and maternal age, umbilical cord blood T lymphocytes, NK cell activity, Low dose of irradiation of cord blood T lymphocytes and NK cell activity changes, cord blood serum colony stimulating substances and clinically used for leukopenia efficacy of experimental and clinical studies. Results Each cord blood accounted for 95.4% in the range of 100-150 ml. There was no correlation between the amount of MNC and MNC, and it was more suitable within 3 days in 6 ℃ refrigerator and the activity of MNC was above 91.2%. The number of CD34 + Sex-related, and other factors without correlation; umbilical cord blood T lymphocytes and NK cell activity is low, low dose irradiation can increase its activity; cord blood serum-rich cell colony-stimulating active substances, mainly IL-3; leukopenia Infusion of cord blood patients can increase the total number of peripheral white blood cells. Conclusion Due to the above advantages, umbilical cord blood has broad prospects for clinical application.