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为观察经UVC照射后的凋亡SMC内Bcl2蛋白表达的情况。应用组织贴块法进行体外SMC培养,SP免疫组织化学技术和图像分析技术检测经UVC照射后的SMC内Bcl2蛋白的表达。结果:UVC照射体外SMC后,细胞出现典型的凋亡形态学和生化指标的改变;凋亡SMC胞浆
To observe the expression of Bcl2 protein in apoptotic SMCs after UVC irradiation. The tissue patch method was used to detect the expression of Bcl2 protein in SMC after UVC irradiation in vitro by SP immunohistochemistry and image analysis. Results: After UVC irradiation in vitro, the cells showed typical morphological changes of apoptosis and biochemical indexes. The apoptotic SMC cytoplasm