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目的 :研究显微分光光度计测量细胞DNA对肿瘤诊断和预后的价值。方法 :应用OPTON 0 3型扫描分光光度计和改良的DNA Feulgen染色方法对 15例涎腺发生的肿瘤、17例恶性淋巴瘤、2 3例早期胃癌和 2 0例食管及贲门早期浅表癌的癌细胞DNA含量进行了测量。测量结果与病理组织学诊断及随访结果进行了对比分析。结果 :癌细胞DNA含量增高 ,胞核DNA含量分布广泛 ,出现非整倍体细胞核。非整倍体型早期食管癌的复发率较二倍体型早期食管癌高 (P <0 0 5 )。结论 :细胞光度术测量细胞DNA含量对鉴别良、恶性肿瘤可以提供客观依据。DNA倍体数对食管癌预后判断有一定价值。本研究建立的DNA Feulgen染色方法效果极佳。
OBJECTIVE: To study the value of measuring the DNA of cells by micro-spectrophotometer in the diagnosis and prognosis of tumors. Methods: 15 cases of salivary gland tumor, 17 cases of malignant lymphoma, 23 cases of early gastric cancer and 20 cases of early superficial esophagus and esophageal cancer were treated with OPTON 0 3 scanning spectrophotometer and modified DNA Feulgen staining. Cancer cell DNA content was measured. The results of the measurement and histopathological diagnosis and follow-up results were compared. Results: DNA content of cancer cells increased, DNA content of the nucleus was widely distributed, and aneuploid nuclei appeared. The recurrence rate of aneuploidy early esophageal cancer was higher than that of early diploid esophageal cancer (P <0.05). Conclusion: Cytometric measurement of cellular DNA content can provide an objective basis for the differential diagnosis of benign and malignant tumors. DNA ploidy on the prognosis of esophageal cancer have some value. The DNA Feulgen staining method established in this study is very effective.