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目的建立酒精性肝损伤大鼠模型,研究大黄酸对酒精性肝损伤大鼠的干预作用。方法健康SD大鼠32只,分为正常对照组、酒精性肝损伤模型组、大黄酸高剂量组和低剂量组。正常对照组采用标准饲料喂养。酒精性肝损伤模型组、大黄酸高剂量组和低剂量组灌胃造模乳液10ml/(kg·d)制备酒精性肝损伤模型。造模的同时,大黄酸高、低剂量组分别灌胃大黄酸80 mg/kg和40 mg/kg干预。每日给药一次,连续8周。末次给药后,测定大鼠血清及肝脏中超氧化物歧化酶(SOD)、丙二醛(MDA)、总胆固醇(TC)、甘油三酯(TG)、天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)等指标。大鼠肝脏HE染色观察病理学变化。结果大黄酸可提高酒精性肝损伤大鼠的抗氧化能力,可使酒精性肝损伤大鼠总胆固醇和甘油三脂的含量降低,可降低酒精性肝损伤大鼠转氨酶的活性。结论大黄酸对酒精性肝损伤大鼠的肝脏有保护作用。
Objective To establish a rat model of alcoholic liver injury and study the effect of rhein on alcoholic liver injury in rats. Methods Thirty-two healthy SD rats were divided into normal control group, alcoholic liver injury model group, rhein high dose group and low dose group. Normal control group fed with standard feed. Alcoholic liver injury model group, rhein high-dose group and low-dose group intragastric injection of emulsion 10ml / (kg · d) preparation of alcoholic liver injury model. At the same time of modeling, rhein acid high and low dose groups were fed with rhein 80 mg / kg and 40 mg / kg, respectively. Administered once daily for 8 weeks. After the last administration, the contents of superoxide dismutase (SOD), malondialdehyde (MDA), total cholesterol (TC), triglyceride (TG) and aspartate aminotransferase (AST) , Alanine aminotransferase (ALT) and other indicators. Rat liver HE staining observed pathological changes. Results Rhein increased the antioxidant capacity of rats with alcoholic liver injury, decreased the content of total cholesterol and triglyceride, and decreased the activity of aminotransferase in rats with alcoholic liver injury. Conclusion Rhein has a protective effect on the liver of alcoholic liver injury rats.