论文部分内容阅读
目的 :研究 6 %羟乙基淀粉 (HES)急性高容量血液稀释 (acute hypervolemic hemodilution,AHH)对缺血再灌注损伤肾 SOD和 MDA的影响。方法 :健康新西兰雄性白兔 2 6只 ,随机分为二组 (n=1 3) :HESAHH组 (AHH组 )和对照组 (C组 )。AHH组于 AHH后、C组于补足丢失液体后 ,用无损伤动脉夹夹闭肾动脉 ,缺血 30分钟 ,松开动脉夹再灌注 1 80分钟 ,制成缺血再灌注损伤模型。于再灌注 1 80分钟时取肾下极肾组织 :作匀浆测 SOD和 MDA。结果 :(1 )稀释后和稀释前相比 ,动物 ECG和 MAP无明显变化 ,HR虽明显降低 ,但在正常范围内 ;CVP从 2 .85± 1 .2 5 cm H2 O升高至 4 .4 8± 1 .6 9cm H2 O,有显著性差异。 (2 )血液稀释后 ,动物血红蛋白从 1 2 7.85± 1 2 .5 5 g/ L下降到 87.85± 7.74 g/ L ,Hct从 37± 2 .1 2 %下降到 2 5± 3.83% ,达中度血液稀释。 (3) SOD测定 ,AHH组比 C组明显升高 ,有显著性差异。(4 ) MDA测定 ,AHH组比 C组明显降低 ,有显著性差异
AIM: To investigate the effects of acute hypervolemic hemodilution of 6% hydroxyethyl starch (HES) on renal SOD and MDA in rats with ischemia-reperfusion injury. Methods: Twenty-six male New Zealand white rabbits were randomly divided into two groups (n = 13): HESAHH group (AHH group) and control group (C group). AHH group after AHH, C group to make up for the loss of fluid, with non-invasive artery clip closed renal artery, ischemia for 30 minutes, release the artery clip and reperfusion 180 minutes, made of ischemia-reperfusion injury model. At 180 minutes after reperfusion, the kidneys under the kidney were taken for determination of SOD and MDA. Results: (1) There was no significant change of ECG and MAP in the animals after dilution compared with that before dilution. Although the HR decreased obviously, CVP increased from 2.85 ± 1.25 cm H 2 O to 4 within the normal range. 4 8 ± 1 .6 9cm H2 O, there are significant differences. (2) After hemodilution, hemoglobin decreased from 12 7.85 ± 12.5 2.5 g / L to 87.85 ± 7.74 g / L and Hct decreased from 37 ± 2.12% to 25 ± 3.83% Degree of hemodilution. (3) SOD assay, AHH group than C group was significantly increased, with significant differences. (4) MDA, AHH group was significantly lower than the C group, there was a significant difference